« Previous Article
Next Article »

Original Article Open Access


Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats

Olaoluwa S. Olukiran, Rufus O. Akomolafe, Kayode D. Bamitale, Akande O. Ajayi, Raphael E. Okonji, Ronald A. Bejide.

Cited by (1)

Abstract
Objective: Livolin forteŽ (LIV) is a hepatoprotective drug that is being used in hospitals worldwide in the treatment and management of liver diseases. However, as it appear through the available published literature, the physiological effects of the drug on the liver using a rat model of hepatotoxicity is not yet well established. This study assessed the curative and prophylactic effects of LIV on carbon tetrachloride (CCl4) induced liver damage in rats.
Methods: Twenty-four adult Wistar rats were randomly divided into four groups: group I (normal control) received 0.3 ml/kg/day of propylene glycol for one month; group II (toxicant control) was given 0.7 ml/kg/day of CCl4 dissolved in olive oil (1:1, v/v) orally for 7 days; group III received 5.2 mg/kg/day of LIV for one month followed by CCl4 for one week; group IV received CCl4 for one week and subsequently LIV (5.2 mg/kg/day) was administered for one month. Half of the rats were sacrificed one day after the last treatment, the other half after a 2-week recovery period.
Results: The toxicant control group had significantly higher AST and ALT activities, total bilirubin and lower total protein and GSH levels compared to the normal control rats. Similarly, significant increase in serum activities of AST and ALT were observed in group III compared to the normal control rats a day after the last treatment with CCl4, whereas after the recovery period no significant differences were observed in nearly all the parameters. Moreover, group IV showed no significant differences in the parameters mentioned above compared to the normal control rats a day after the last treatment with LIV and after the recovery period.
Conclusion: The results of this study indicated that LIV was better as a curative agent rather than a prophylactic agent in rats.

Key words: Carbon tetrachloride; Curative; Livolin forteŽ; Prophylactic; Rat


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Export to
Export to
Related Records
 Articles by Olaoluwa S. Olukiran
Articles by Rufus O. Akomolafe
Articles by Kayode D. Bamitale
Articles by Akande O. Ajayi
Articles by Raphael E. Okonji
Articles by Ronald A. Bejide
on Google
on Google Scholar
Article Statistics
 Viewed: 42803
Downloaded: 710
Cited: 1

REFERENCES
1.Subramoniam A, Pushpangadan P. Development of phytomedicine for liver diseases. Indian J Pharmacol 1999; 31:166-75.


2.Baranisrinivasan P, Elumalai EK, Sivakumar C, Theresa SV, David E. Hepatoprotective effect of Enicostemma Littorale blume and Eclipta alba during ethanol induced oxidative stress in albino rats. Inter J Pharmacol 2009; 5:268-72.

http://dx.doi.org/10.3923/ijp.2009.268.272


3.Marina N. Hepatotoxicity of antiretroviral: incidence, mechanisms and management. J Hepatol 2006; 44:S132-9.

http://dx.doi.org/10.1016/j.jhep.2005.11.027

[
Pubmed]   


4.Poongothai K, Karkuzhali G, Siva-Prakash T, Sangeetha T, Saravanan G, Deepa R, Gopalakrishnan S, Mohan V. Effect of essentiale in diabetic subjects with non-alcoholic fatty liver. Int J Diab Dev Countries 2005; 25:1-6.


5.Ovchinnikov AA, Tsvettsikh VE, Berdichevskii BA, Lykov VN, Suktanbaev VR, Murychev, AV. Essential phospholipids in the treatment of chronic pyelonephritis. Urol Nefrol (Mosk) 1996; 4:7-9.

[
Pubmed]   


6.Poo JL, Feldmann G, Erlinger S, Braillon A, Gaudin C, Dumont M, Lebrec D. Ursodeoxycholic acid limits liver histologic alterations and portal hypertension induced by bile duct ligation in the rat. Gastroenterology 1992; 102:1752-9.

[
Pubmed]   


7.Guicciardi ME, Gores GJ. Is ursodeoxycholate an antiapoptic drug? Hepatology 1998; 28:1721-3.

http://dx.doi.org/10.1002/hep.510280639

[
Pubmed]   


8.Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72:248-54.

http://dx.doi.org/10.1016/0003-2697(76)90527-3

[
Pubmed]   


9.Tietze F. Enzymatic method for the quantative determination of nanogram amount of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 1969; 27:502-22.

http://dx.doi.org/10.1016/0003-2697(69)90064-5

[
Pubmed]   


10.Cabre M, Camps J, Paternain JL, Ferre N, Joven J. Time- course of changes in hepatic lipid peroxidation and glutathione metabolism in rats with carbon tetrachloride- induced cirrhosis. Clin Exp Pharmacol Physiol 2000; 27:694-9.

http://dx.doi.org/10.1046/j.1440-1681.2000.03322.x

[
Pubmed]   


11.Kandasamy CS, Shimna TP, Mohammed BE, Arul RP, Gopal V, Venkatnarayanan R. Anti-hepatotoxic activity of polyherbal formulation in carbon tetrachloride induced toxicity in rats. RJPBCS 2010; 1:341-6.


12.Kew MC. Serum aminotransferase concentration as evidence of hepatocellular damage. Lancet 2000; 355:591-2.

http://dx.doi.org/10.1016/S0140-6736(99)00219-6

[
Pubmed]   


13.Wallnoefer H, Hanusch M. Essential phospholipids in the treatment of hepatic disease. Med Monatsschr 1973; 27:131-6.


14.Bun SS, Bun H, Guedon D, Rosier C, Ollivier E. Effect of green tea extract on liver functions in Wistar rats. Food Chem Toxicol 2006; 44:1108-13.

http://dx.doi.org/10.1016/j.fct.2006.01.006

[
Pubmed]   


15.Guyton AC, Hall JE. Textbook of Medical Physiology. Harcourt International Edition, 10th edition, W.B. Saunders, Philadelphia, PA, pp 734-862, 2001.


16.Naganna B. Plasma proteins. In: Talwar GP, Srivastava LM, Moudgil KD (eds) Textbook of Biochemistry and Human Biology, 2nd edition, Prentice-Hall of India, New-Delhi, pp 59-61, 1989.


17.Li Z, Agellon LB, Allen TM, Umeda M, Jewell L, Mason A, Vance DE. The ratio of phosphatidylcholine to phosphatidylethanolamine influences membrane integrity and steatohepatitis. Cell Metab 2006; 3:321-31.

http://dx.doi.org/10.1016/j.cmet.2006.03.007

[
Pubmed]   


18.Brook JG, Linn S, Aviram M. Dietary soya lecithin decreases plasma triglyceride levels and inhibits collagen- and ADP- induced platelet aggregation. Biochem Med Metab Biol 1986; 35:31-9.

http://dx.doi.org/10.1016/0885-4505(86)90055-1

[
Pubmed]   


19.Major LF, Goyer PF. Effects of disulfiram and pyridoxine on serum cholesterol. Ann Intern Med 1978; 88:53-6.

http://dx.doi.org/10.7326/0003-4819-88-1-53

[
Pubmed]   


20.Lii CK, Ko YJ, Chiang MT, Sung WC, Hen HW. Effect of dietary vitamin E on antioxidant status and antioxidant enzyme activities in Sprague-Dawley rats. Nutr Cancer 1998; 32:95-100.

http://dx.doi.org/10.1080/01635589809514725

[
Pubmed]   


21.Brigelius-Flohe R. Vitamin E: the shrew waiting to be tamed. Free Radic Biol Med 2009; 46:543-54.

http://dx.doi.org/10.1016/j.freeradbiomed.2008.12.007

[
Pubmed]   


This Article Cited By the following articles

Aqueous extract of Monodora myristica ameliorates cadmium-induced hepatotoxicity in male rats
SpringerPlus 2016; 5(1): .

1
 

How to Cite this Article
Pubmed Style

Olukiran OS, Akomolafe RO, Bamitale KD, Ajayi AO, Okonji RE, Bejide RA. Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. J Exp Integr Med. 2014; 4(1): 57-65. doi:10.5455/jeim.310813.or.088



Web Style

Olukiran OS, Akomolafe RO, Bamitale KD, Ajayi AO, Okonji RE, Bejide RA. Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. www.scopemed.org/?mno=40607 [Access: August 18, 2017]. doi:10.5455/jeim.310813.or.088



AMA (American Medical Association) Style

Olukiran OS, Akomolafe RO, Bamitale KD, Ajayi AO, Okonji RE, Bejide RA. Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. J Exp Integr Med. 2014; 4(1): 57-65. doi:10.5455/jeim.310813.or.088



Vancouver/ICMJE Style

Olukiran OS, Akomolafe RO, Bamitale KD, Ajayi AO, Okonji RE, Bejide RA. Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. J Exp Integr Med. (2014), [cited August 18, 2017]; 4(1): 57-65. doi:10.5455/jeim.310813.or.088



Harvard Style

Olukiran, O. S., Akomolafe, R. O., Bamitale, K. D., Ajayi, A. O., Okonji, R. E. & Bejide, R. A. (2014) Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. J Exp Integr Med, 4 (1), 57-65. doi:10.5455/jeim.310813.or.088



Turabian Style

Olukiran, Olaoluwa S., Rufus O. Akomolafe, Kayode D. Bamitale, Akande O. Ajayi, Raphael E. Okonji, and Ronald A. Bejide. 2014. Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. Journal of Experimental and Integrative Medicine, 4 (1), 57-65. doi:10.5455/jeim.310813.or.088



Chicago Style

Olukiran, Olaoluwa S., Rufus O. Akomolafe, Kayode D. Bamitale, Akande O. Ajayi, Raphael E. Okonji, and Ronald A. Bejide. "Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats." Journal of Experimental and Integrative Medicine 4 (2014), 57-65. doi:10.5455/jeim.310813.or.088



MLA (The Modern Language Association) Style

Olukiran, Olaoluwa S., Rufus O. Akomolafe, Kayode D. Bamitale, Akande O. Ajayi, Raphael E. Okonji, and Ronald A. Bejide. "Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats." Journal of Experimental and Integrative Medicine 4.1 (2014), 57-65. Print. doi:10.5455/jeim.310813.or.088



APA (American Psychological Association) Style

Olukiran, O. S., Akomolafe, R. O., Bamitale, K. D., Ajayi, A. O., Okonji, R. E. & Bejide, R. A. (2014) Protective and curative effects of Livolin forteŽ on carbon tetrachloride-induced liver damage in Wistar rats. Journal of Experimental and Integrative Medicine, 4 (1), 57-65. doi:10.5455/jeim.310813.or.088